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Molecular Principles of development
- Summary • 60 pages • 2021
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Summary of the course principles of molecular biology. Information collected from the lectures, Q&A's, Computer practicals and book 'Principles of Development'.
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Summary lectures Molecular Principles Of Development (NWI-BB084B)
- Summary • 58 pages • 2021
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This is a summary of all lectures in the course.
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Summary lectures 'Molecular principles of development'
- Summary • 65 pages • 2021
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A summary of all the given lectures of Principles of Development in 2020-2021. The summary contains pictures from the slides and from the book.
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15. Genomic Imprinting
- Class notes • 8 pages • 2020
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- To give an overview of genomic imprinting, when it is initiated and how imprinting disorders can arise
- Describe key events in X-inactivation including the role of the Xist non-coding RNA
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10. Germ Layer Specification
- Class notes • 8 pages • 2020
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- Discuss the evidence for the nodal morphogen acting during mesoderm induction.
- Discuss the concept of the vertebrate dorsal organiser.
- Discuss the evidence that the brachyury transcription factor is a conserved regulator of germ layer specification.
- Draw conclusions regarding the molecular pathways regulating development of Urbilateria.
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13. Histone Modifications
- Class notes • 9 pages • 2020
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- Provide an overview of the polycomb and trithorax group complexes and their activities
- Describe evidence that indicates stem cell chromatin is in an open ‘permissive’ state
- Understand what is bivalent chromatin and why it may be important for stem cell biology and development
- Understand the steps of chromatin immunoprecipitation and why it is a useful technique in epigenetic studies
- Describe the Nodal signalling example and experimental approach, and understand how it illustrates ...
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6. Limb Development
- Class notes • 7 pages • 2020
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- Identify the important signalling centres in the developing limb bud and describe the experiments that identified these centres and demonstrated their importance.
- Describe the experimental evidence for the role of FGF signalling in limb bud outgrowth
- Describe the experimental evidence that shows that Shh acts as a morphogen to pattern the anterior-posterior axis of the limb bud.
- Describe how the important signalling centres in the limb bud co-ordinately regulate pattern formation in the ...
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3. Neural Induction
- Class notes • 6 pages • 2020
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- Appreciate and describe the molecular mechanisms underlying neural induction
- Describe TGF-beta (TGF-β) signal transduction and the differences between the BMP versus nodal pathways
- Discuss the experiments that demonstrate a role for BMP antagonists in vertebrate neural induction
- Describe how antisense morpholino oligos can be used to inhibit gene function
- Appreciate and describe the differences between the processes of neural induction versus neurogenesis
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1. Segmentation Genes
- Class notes • 7 pages • 2020
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-Describe the hierarchy of gene activity that regulates Drosophila segmentation
-Distinguish and explain methods used to investigate protein vs mRNA expression
-Describe the syncytial nature of the early stages of fly development and understand its significance.
-Understand and describe at the molecular level how the Gap genes regulate the expression of Eve stripe 2
-Make predictions about segment polarity gene expression in a pair-rule mutant embryo or about pair-rule gene expression in a Gap m...
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11. Small Changes Big Differences
- Class notes • 10 pages • 2020
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- Understand how the neural crest contributes to cardiac development and the pathology underlying congenital cardiac abnormalities.
- Explain how certain SNPs might contribute to congenital abnormalities.
- Discuss the evidence that Notch signalling is important during cardiac morphogenesis